These citations are obtained from the National Library of Medicine's PubMed Database View in PubMed Rethinking your purification procedure. Methods Enzymol. 2009;463:809-11 Authors: Deutscher MP PMID: 19892203 [PubMed - in process] View in PubMed Affi-Gel Blue for nucleic acid removal and early enrichment of nucleotide binding proteins. Methods Enzymol. 2009;463:343-5 Authors: Deutscher MP Passage of an extract or supernatant fraction through a column of Affi-Gel Blue and batchwise elution can be a rapid and effective early procedure for removal of nucleic acid, concentration of the sample and purification of nucleotide binding proteins. PMID: 19892181 [PubMed - in process] View in PubMed Tagging for protein expression. Methods Enzymol. 2009;463:239-58 Authors: Malhotra A Tags are frequently used in the expression of recombinant proteins to improve solubility and for affinity purification. A large number of tags have been developed for protein production and researchers face a profusion of choices when designing expression constructs. Here, we survey common affinity and solubility tags, and offer some guidance on their selection and use. PMID: 19892176 [PubMed - in process] View in PubMed Maintaining protein stability. Methods Enzymol. 2009;463:121-7 Authors: Deutscher MP Proteins are fragile molecules that often require great care during purification to ensure that they remain intact and fully active. Nowadays, many proteins are also purified in small amounts under denaturing conditions by various gel electrophoretic techniques, such that inactive proteins are obtained. But even here, it is usually advantageous to maintain the protein in an intact form. In the case of enzymes, and other proteins with assayable biological activities, maintenance ... View in PubMed Measurement of enzyme activity. Methods Enzymol. 2009;463:57-71 Authors: Harris TK, Keshwani MM To study and understand the nature of living cells, scientists have continually employed traditional biochemical techniques aimed to fractionate and characterize a designated network of macromolecular components required to carry out a particular cellular function. At the most rudimentary level, cellular functions ultimately entail rapid chemical transformations that otherwise would not occur in the physiological environment of the cell. The term enzyme is used to ... View in PubMed Setting up a laboratory. Methods Enzymol. 2009;463:37-42 Authors: Deutscher MP PMID: 19892165 [PubMed - in process] View in PubMed Adaptation versus Allometry: Population and Body Mass Effects on Hypoxic Metabolism in Fundulus grandis. Physiol Biochem Zool. 2009 Nov 5; Authors: Everett MV, Crawford DL Abstract Hypoxia has significant effects on organisms, from metabolic reduction to death, and could be an important evolutionary force affecting the variation among populations within a species. To determine intraspecific variation in hypoxic metabolism and the effect of body mass, we examine rates of oxygen consumption ([Formula: see text]) at seven oxygen concentrations among seven ... View in PubMed Calcium hydroxylapatite (Radiesse): safety, techniques and pain reduction. J Drugs Dermatol. 2009 Oct;8(10 Suppl):s21-3 Authors: Busso M Although the cosmetic applications of injectable calcium hydroxylapatite (CaHA) commenced in the first years of the 21st century, only recently has the clinical use of this filler been optimized. This update will review safety, techniques, pain reduction by mixing with lidocaine and the use of CaHA in ethnic skin. PMID: 19891118 [PubMed - in process] View in PubMed Optimizing management strategies in patients with chronic hepatitis B. Introduction. Am J Med. 2008 Dec;121(12 Suppl):S1-2 Authors: Schiff ER PMID: 19185069 [PubMed - in process]   View in PubMed Related Articles A critical analysis of the tumour immunosurveillance controversy for 3-MCA-induced sarcomas. Br J Cancer. 2009 Aug 4;101(3):381-6 Authors: Schreiber TH, Podack ER The cancer immunoediting hypothesis has gained significant footing over the past decade as a result of work performed using sarcomas induced by 3-methylcholanthrene (3-MCA) in mice. Despite the progress made by several groups in ... View in PubMed Related Articles Preventing Hearing Damage Using Topical Dexamethasone During Reversible Cochlear Ischemia: An Animal Model. Otol Neurotol. 2009 Jul 25; Authors: Morawski K, Telischi FF, Bohorquez J, Niemczyk K HYPOTHESIS:: Local application of dexamethasone to the round window (RW) niche prevents cochlear damage caused by local reversible ischemia. BACKGROUND:: Cochlear ischemia induced by internal auditory ... View in PubMed Related Articles Mass Clamping of the Hilum to Facilitate Difficult Hepatectomy During Liver Transplantation: A Single Center 10 Year Experience. Ann Surg. 2009 Jul 27; Authors: Pararas N, Levi D, Selvaggi G, Moon J, Nishida S, Tryphonopoulos P, Island E, Tzakis A INTRODUCTION:: Classic dissection of the hilar structures during the hepatectomy portion of the liver transplant procedure is sometimes extremely ... View in PubMed Related Articles Peer victimization and social anxiety in adolescents: prospective and reciprocal relationships. 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